摘要:This study aims at investigating the practices and sexual behaviors in a university population since positive behaviors may develop best abilities for the prevention of high-risk behaviors, major sexual satisfaction and a more meaningful life. This was a cross-sectional study, which involved students from all programs of the Universidad de Manizales (Caldas, Colombia), who were between first and ninth semester. The sample of this study was 267 students of a population of 3600 regular students, which was obtained by probability sampling stratified by gender and program. The average age was 21.69, with a higher ratio of women (58.8%), 1.5% were homosexual and 5% bisexual; the most used contraception method was barrier methods (41.3%); 15.2% had had some kind of sexual transmission infection (STI). Regarding sexual practices 22.8% performed the different practices (masturbation, oral sex, anal sex, vaginal sex), and the average age of first sexual intercourse was 16.09; 53.1% agrees with abortion and 84% agreed with its total decriminalization; 73.2% have an acceptance and tolerance of homosexuality. The study allowed seeing the influence of several variables such as gender, family, religion and educational background on behavior and responsible sexual practice.