The methanol extract of the roots and stems of Daphne genkwa and its constituents yuanhuacin ( 1 ) and genkwanine N were previously reported to have Nurr1 activating effects and neuroprotective effects in an animal model of Parkinson’s disease (PD). In this study, four more daphnane-type diterpenes (acutilonine F ( 2 ), wikstroemia factor M₁ ( 3 ), yuanhuadine ( 5 ), and yuanhuatine ( 6 )) and two phorbol-type diterpenes (prostratin Q ( 4 ) and 12- O - n -deca-2,4,6-trienoyl-phorbol-(13)-acetate ( 7 )) were isolated as Nurr1 activating compounds from the D. genkwa extract. Consistent with their higher Nurr1 activating activity, compounds 1 , 4 , 5 , and 7 exhibited higher inhibitory activity on lipopolysaccharide (LPS)-induced nitric oxide (NO) production in murine microglial BV-2 cells with an IC₅₀ (µM) of 1–2, which was 15–30 times more potent than that of minocycline (29.9 µM), a well-known anti-neuroinflammatory agent. Additionally, these diterpenes reduced expression and transcription of LPS-induced pro-inflammatory cytokines in BV-2 cells. Thus, the daphnane-type and phorbol-type diterpenes had anti-neuroinflammatory activity with Nurr1 activation and could be responsible for the anti-PD effect of the roots and stems of D. genkwa .