Two new secoiridoid glycosides, obtusifolisides A and B ( 1 , 2 ), together with 7 known secoiridoid glycosides ( 3 – 9 ) were isolated from the twigs of Ligustrum obtusifolium . The chemical structures of new compounds were determined by a spectroscopic data analysis, including one and two dimensional (1D-, 2D)-NMR, High resolution-MS, and experiments involving chemical reactions. The isolated secoiridoid glycosides were evaluated for their anti-inflammatory effects in lipopolysaccharide (LPS)-stimulated BV-2 murine microglia cells. Compounds 2 , 5 , 6 , 8 , and 9 significantly reduced the production of nitric oxide (NO), with IC₅₀ values of 5.45, 11.17, 14.62, 15.45, and 14.96 µM, respectively. None of the compounds were toxic to the cells. Additionally, we evaluated the neuroprotective effects of compounds 1 – 9 on nerve growth factor (NGF) induction in a C6 rat glioma cell line. Compounds 2 and 6 upregulated NGF secretion to 155.56±7.16%, and 139.35±11.65%, respectively, without significant cell toxicity.