Cyprotuoside C ( 1 ) and cyprotuoside D ( 2 ), two new cycloartane glycosides were isolated from the ethanol extract of the rhizomes of Cyperus rotundus . Their structures were identified as 24 R -9,10- seco -cycloartan-1(10),9(11)-dien-3β,7β,24,25-tetraol 3- O -β-D-xylopyranosyl-(1→4)-[α-L-arabinopyranosyl-(1→6)]-β-D-glucopyranosyl-25- O -β-D-glucuronide ( 1 ) and 9,10- seco -cycloartan-1(10),9(11),23(24)-trien-3β,7β,25-triol 3- O -β-D-xylopyranosyl-(1→4)-{α-L-arabinopyranosyl-(1→6)-[α-L-rhamnopyranosyl-(1→2)]}-β-D-glucopyranosyl-25- O -β-D-glucuronide ( 2 ) by spectroscopic methods.