标题：Transduction of the vitellogenic signal of juvenile hormone by Methoprene-tolerant in the cockroach Blattella germanica (L.) (Dictyoptera, Blattellidae)
出版社：International Academy of Ecology and Environmental Sciences
摘要：In adult females of the cockroach Blattella germanica, juvenile hormone (JH) promotes vitellogeninproduction. Depletion of Methoprene-tolerant (Met) mRNA levels with RNAi resulted in a clear reduction ofvitellogenin expression in the fat body while basal oocyte growth was impaired. This demonstrates that Met isessential to transduce the vitellogenic signal of JH in this species. Interestingly, the expression of thetranscription factor Kruppel homolog 1 (Kr-h1) was also reduced in Met-depleted specimens. This indicatesthat the JH signaling pathway promotes the transcription of Kr-h1 in adult fat body tissues, as occurs in theepidermis during nymphal development. Treatments with JH show that the expression of Met does not dependon JH, which suggests that Met is upstream the JH signaling pathway acting as JH receptor, as reported inother models and processes, especially in metamorphosis. JH treatments increased the transcription levels ofvitellogenin and Kr-h1, which again suggests that Kr-h1 is a JH-dependent transcription factor in the fat bodyof adult females. The important roles of Met in nymphal development, as previously reported, and thosereported herein in relation to reproduction, suggests that it can be an interesting target for the control of B.germanica in urban environments, using RNAi approaches.
