出版社:International Academy of Ecology and Environmental Sciences
摘要:The soluble proteins from nymphs and adults of Sogatella furcifera were analyzed by SDS-PAGE. Thenumber of protein bands increased gradually as the nymphs developing, such as six and 14 protein bands werefound in 3rd-instar nymphs and 5th-instar nymphs respectively. At the same time, we found that three bandsexpressed in each instar, two bands began to appear from 4th-instar, and six bands were specific in 5th-instar.There were four bands that their content in 5th-instar nymphs with long-winged disc was at least 65.61% higherthan in 5th-instar nymphs with short-winged disc. There were 13 protein bands observed in male adults, whilefemale adults had 13 corresponding protein bands and a specific band expressed only in tissue. Comparingbetween two wing-type adults, four bands were specific to long-winged adults, while the content of other threebands in long-winged adults was at least 72.54 % higher than in short-winged adults. Finally, these specificprotein bands associated with wing or sex were discussed what kind role they played in wing or sexualdifferentiation. The results will be helpful to further explore the mechanism of wing or sexual differentiationabout planthoppers.
关键词:SDS-PAGE; Sogatella furcifer; soluble protein; wing differentiation; sexual differentiation.