期刊名称：Annals of Agricultural and Environmental Medicine
印刷版ISSN：1232-1966
电子版ISSN：1898-2263
出版年度：2018
卷号：25
期号：1
页码：157-161
DOI：10.26444/aaem/74406
出版社：Institute of Agricultural Medicine in Lublin
摘要：Introduction. Phenol and 1-hydroxypyrene are biological markers of exposure to polycyclic aromatic hydrocarbons (PAH)that have certain negative effects on parenchymal organs such as the human placenta. The literature presents only fewreports regarding the effects of elevated PAH levels on the functions of the human placenta.Objective. The aim of the work is to assess the effects of elevated PAH levels in excreted urine on the endocrine andmetabolic functions of the human placenta obtained from a normal pregnancy.Material and methods. Tissue material from 50 afterbirths from Płock constituted a study group, whereas 50 afterbirthsfrom Kutno constituted a control group. Immunohistochemical reactions with the peroxidase method using LSAB kits (DAKO,Denmark) were performed. The extent and intensity of reactions were analysed. The levels of phenols and 1-hydroxypyrenein the excreted urine of pregnant women (undergoing delivery) were detected using gas chromatography and colorimetry.The statistical analysis used the PQStat v.1.6.2 software; moreover, t-student and chi-square tests were used. Differenceswere considered to be significant at the significance level of 95% (p<0.05).Results. The levels of phenol and 1-hydroxypyrene in the excreted urine were demonstrated to be statistically significantlyhigher in patients living in the area of Płock. Statistically lower expression of placental glutathione transferase and lowerimmunohistochemical demonstration of the placental phosphatase activity were observed in placentas from Płock. It hasbeen demonstrated that the expression of the oestrogen receptor activity and placental gonadotropin is significantly higherin placentas from areas not contaminated with aromatic hydrocarbons (Kutno).Conclusions. The course of pregnancy in the environment with elevated levels of aromatic hydrocarbons leads to impairedplacental functioning and reduced endocrine and metabolic activity of the placenta.
关键词：Human placenta; Endocrine and Metabolic functions; Aromatic hydrocarbons; Phenol; 1-hydroxypiren
