期刊名称：Annals of Agricultural and Environmental Medicine
印刷版ISSN：1232-1966
电子版ISSN：1898-2263
出版年度：2017
卷号：24
期号：4
页码：618-623
DOI：10.5604/12321966.1235159
出版社：Institute of Agricultural Medicine in Lublin
摘要：Introduction. Composite materials used in dentistry indicate adverse biological effects in laboratory conditions. One reasonfor this activity is incomplete conversion of their polymer matrix, favoring chemical instability and release of biologicallyharmful components to the external environment.Aim. The aim of the study was to assess the degree of conversion of restorative materials commonly available on theEuropean market and to examine the cytotoxic effects of their eluates in vitro.Material and methods. Using the Fournier transform infrared spectroscopy (FTIR) technique of analysis, the degree ofpolymer matrix conversion of 6 restorative materials was examined: Gradia Direct, Arkon, Filtek Z550, Herculite XRV, TetricEvo Ceram, Charisma, polymerized with LED light. In order to assess the cytotoxicity of eluates of the studied materialsobtained after 1 hour, 24 hours and 7 days, the MTT assay was used in cultured 3T3 cells. The results were statisticallyanalyzed at significance level of p=0.05.Results. The conversion degree of the assessed polymers ranged from 31.56% for Tetric Evo Ceram to 75.84% for Arcon.The strongest (p=0.05) cytotoxic effect was demonstrated after 7-day observation of Tetric Evo Ceram eluates, reducing themetabolic activity of cells down to 56%. A positive correlation (r(x, y)=0.62) between the degree of conversion of compositematerials and cytotoxic effects of their eluates on cell cultures was confirmed.Conclusions.1. Restorative dental materials are chemically unstable in the conditions of the present study.2. Polymer-based restorative dental materials available on the European market demonstrate cytotoxic properties constitutinga potential threat to the patients’ health.
关键词：dental composites; cytotoxicity; degree of conversion
