期刊名称：Annals of Agricultural and Environmental Medicine
印刷版ISSN：1232-1966
电子版ISSN：1898-2263
出版年度：2017
卷号：24
期号：3
页码：366-371
DOI：10.26444/aaem/75688
出版社：Institute of Agricultural Medicine in Lublin
摘要：Introduction and objective. Aleutian Disease is a significant biological factor causing substantial losses in mink farming.The virus inducing the disease also infects wild populations which may constitute an asymptomatic reservoir. To comparegenetic variants of the AMD virus occurring in wild and farmed mink populations, an analysis was performed on a fragmentof the VP2 protein sequence of the virus infecting both populations, taken from different living environments.Material and method. Genetic material was isolated from 11 farmed animals in which anti-AMDV antibodies had beendetected and from 20 wild animals. The DNA obtained was amplified using primers specific for the fragment encoding theVP2 protein. The product obtained was sequenced and bioinformatic analysis was performed.Results. Viral material was detected in 11 farmed and 7 free-living animals. Similarity of sequences averaged 99% withingroups and 94% between groups. The sequencing results made it possible to identify characteristic changes for eachgroup. In the isolates from the wild animals, the following changes were observed in the epitope region with respect tothe reference sequence: C3704T, G3710A, T3722C, T3746C and A3749G. In the isolates from the farmed animals a G3779Atransition was noted. Phylogenetic analysis showed that the variants infecting the two groups occupy separate branchesof the phylogenetic tree.Conclusion. The variants of the virus infecting the two groups may have a common origin, but at present they constitutetwo separate groups, with characteristic differences making it possible to recognize their genotype
