首页    期刊浏览 2024年11月30日 星期六
登录注册

文章基本信息

  • 标题:Kinetics of plasma apolipoprotein E isoforms by LC-MS/MS: a pilot study
  • 作者:Valentin Blanchard ; Stéphane Ramin-Mangata ; Stéphanie Billon-Crossouard
  • 期刊名称:JLR Papers In Press
  • 印刷版ISSN:0022-2275
  • 电子版ISSN:1539-7262
  • 出版年度:2018
  • 卷号:59
  • 期号:5
  • 页码:892-900
  • DOI:10.1194/jlr.P083576
  • 语种:English
  • 出版社:American Society for Biochemistry and Molecular Biology
  • 摘要:Human apoE exhibits three major isoforms (apoE2, apoE3, and apoE4) corresponding to polymorphism in the APOE gene. Total plasma apoE concentrations are closely related to these isoforms, but the underlying mechanisms are unknown. We aimed to describe the kinetics of apoE individual isoforms to explore the mechanisms for variable total apoE plasma concentrations. We used LC-MS/MS to discriminate between isoforms by identifying specific peptide sequences in subjects (three E2/E3, three E3/E3, and three E3/E4 phenotypes) who received a primed constant infusion of 2H3-leucine for 14 h. apoE concentrations and leucine enrichments were measured hourly in plasma. Concentrations of apoE2 were higher than apoE3, and concentrations of apoE4 were lower than apoE3. There was no difference between apoE3 and apoE4 catabolic rates and between apoE2 and apoE3 production rates (PRs), but apoE2 catabolic rates and apoE4 PRs were lower. The mechanisms leading to the difference in total plasma apoE concentrations are therefore related to contrasted kinetics of the isoforms. Production or catabolic rates are differently affected according to the specific isoforms. On these grounds, studies on the regulation of the involved biochemical pathways and the impact of pathological environments are now warranted.
  • 关键词:peptide ; liquid chromatography ; tandem mass spectrometry ; stable isotope tracers ; lipoprotein/kinetics ; lipoprotein/metabolism
Loading...
联系我们|关于我们|网站声明
国家哲学社会科学文献中心版权所有