标题：Influence of Glycine, Succinate, Levulinic Acid and Glutamate Concentrations on Growth of Purple Non-Sulfur Photosynthetic Bacteria and 5-Aminolevulinic Acid Production
期刊名称：International Journal of Innovative Research in Science, Engineering and Technology
印刷版ISSN：2347-6710
电子版ISSN：2319-8753
出版年度：2018
卷号：7
期号：4
页码：3839
DOI：10.15680/IJIRSET.2018.0704094
出版社：S&S Publications
摘要：Purple non-sulfur photosynthetic bacteria (PNSB) and their product 5-aminolevulinic acid (5-ALA) havebroad usage in different fields of medicine, agriculture, bioremediation, etc. As demand for aminolevulinic acid isincreasing continuously, new bio-processes and new producers of 5-ALA are researched and optimized. Strain ofpurple non-sulfur bacteria Rhodobacter azotoformans isolated from mineral waters of Armenia is potential producer of5-aminolevulinic acid. For better optimization of 5-ALA production, set of experiments was conducted in order todetermine influence of glycine, succinate, levulinic acid and glutamate on the growth and production of 5-ALA. Asaddition of mentioned chemical compounds in nutrient media is needed for promotion of 5-ALA production, it wastested what is the optimal concentration of glycine, succinate, levulinic acid and glutamate during cultivation to obtainhighest yields. From the results it can be concluded that maximal 5-ALA concentration was achieved in batchcultivation when concentration of glycine was 40 mM, succinate 30 mM, levulinic acid 15 mM and concentration ofglutamate 4g/l. Concentration of 5-ALA was in this case 385 mg/L. Addition of glycine only yielded lowerconcentrations (183.75 mg/L). Concentrations of added glycine and succinate above 40 mM didn’t yield higherconcentrations of 5-ALA. Increase of glutamate concentration above 4 g/L also doesn’t increase 5- ALA yields.
