期刊名称：International Journal of Innovative Research in Science, Engineering and Technology
印刷版ISSN：2347-6710
电子版ISSN：2319-8753
出版年度：2017
卷号：6
期号：2
页码：1697
DOI：10.15680/IJIRSET.2017.0602039
出版社：S&S Publications
摘要：Mostly fish cannot survive more than few hours out of the water, but in the Heteropneustes fossilissurvives up to 60-70hrs under water deprived condition. Such fishes contain high level of ammonia in gills that mayinduce ammonia-mediated toxicity. Therefore, it has been intended to analyze oxidative and high ammonia stressmarkers in brain and liver of Heteropneustes fossilis after aerial exposure. The experimental group of Heteropneustesfossilis was exposed to air and tissues were collected at Zero hour as control and after 20 hrs as experimental. Superoxide dismutase and catalase were selected as oxidative stress markers, lactate dehydrogenase was evaluated forbioenergetics and expression of arginase was analysed through reverse-transcriptase-polymerase chain reaction attranscript level and by enzyme assay at the protein level. The affect of aerial exposure on the protein profile of controland aerial exposed liver and brain of fish were also evaluated by SDS-PAGE followed by MALDI-MS/MS. After 20hours of aerial exposure, the expression of mRNA of super oxide dismutase, and catalase was up-regulated whilelactate dehydrogenase, arginase-I, and arginase-II were down-regulated than the control in the liver as well as the brain.The activities of Cu-Zn-super oxide dismutase and catalase were decreased while Mn- super oxide dismutase, lactatedehydrogenase, malate dehydrogenase, arginase-I, and arginase-II were increased after aerial exposure in liver but thelevel of all enzymes was upregulated in the brain as compared to control. The increased intensity of band of 40kDa and25kDa in liver and 25kDa in the brain that was identified as proteins either associated with actin or myosin orapoptosis. The enzymatic and proteomic analysis confirms that aerial exposure has the prominent effect on the cellularorganization and cytoskeleton formation and maintenance by induction ammonia level followed by ROS.
