Twenty-three selected advanced spring wheat (Triticum aestivum L.) lines from Ottawa Research and Development Centre (ORDC) were compared with four known cultivars for agronomic performance at eight sites in 2016 (Ottawa CEF-C1, Ottawa CEF-C2, St. Isidore, Harrington, Palmerston, Princeville, Kincardine, Beloeil) in Eastern Canada, and for fusarium head blight (FHB). The reaction of these lines to six races of LR was determined in a growth cabinet and the LR susceptible cultivar ‘Morocco’ was included as the control for disease development in these trials. The majority of the selected lines showed no significant differences compared to four check cultivars, however ECSW05 and ECSW48, showed higher yield, moderate resistance to FHB and resistance to most of the tested LR races. Lines ECSW05 and ECSW48 will be advanced to grower’s trials in eastern Canada in 2018 and may be used as sources of resistance to LR for future cultivar development in Eastern Canada.