摘要：The fungi of the genus Fusarium occur worldwide mainly on cereal grains. They are considered to be not only plant-pathogenic, but also the producers of secondary metabolites – mycotoxins. In our study, we focused on the analysis by Q-PCR of two genes expression – Tri4 , Tri10 – involved in the production of Fusarium mycotoxin deoxynivalenol. Reference genes β-tub and UBC were found to be suitable for the expression level normalisation using the program geNorm . The level of expression of the target genes was analysed on four isolates of Fusarium graminearum grown in vitro . In all four isolates, Tri10 gene expression level was lower than that of Tri4 gene expression.
