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  • 标题:Identification of Inhibitors of Phosphate-activated Glutaminase for the Pharmacotherapy of Schizophrenia
  • 本地全文:下载
  • 作者:Andra Mihali ; Cristina Sorrento ; Francine Katz
  • 期刊名称:Columbia Undergraduate Science Journal
  • 印刷版ISSN:1932-765X
  • 电子版ISSN:1932-7641
  • 出版年度:2012
  • 卷号:6
  • 期号:2012
  • 语种:English
  • 出版社:Columbia Undergraduate Science Journal
  • 摘要:Abstract In the brain, phosphate-activated glutaminase catalyzes the recycling of glutamine back to glutamate for excitatory neurotransmission. In mice, genetic knockdown of phosphate-activated glutaminase has been shown to confer resilience to schizophrenia-like symptoms, suggesting that inhibition of glutaminase may have therapeutic potential for the pharmacotherapy of schizophrenia. As there are no known neuroactive inhibitors of glutaminase, i.e. inhibitors that get into the brain, high-throughput screening of a library of 58,000 neuroative compounds was conducted using a fluorescence-based assay; this screen identified 320 potential glutaminase inhibitors. A secondary screen was carried out to access specificity; this yielded 10 hits. Using a kinetic analysis, two leads with low micromolar activity were found.
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