首页    期刊浏览 2024年11月09日 星期六
登录注册

文章基本信息

  • 标题:In vitro models of carcinogenesis: expression of recessive genes by chromosomal mutations.
  • 作者:J B Little
  • 期刊名称:Environmental Health Perspectives
  • 印刷版ISSN:0091-6765
  • 电子版ISSN:1552-9924
  • 出版年度:1989
  • 卷号:81
  • 页码:63
  • 语种:English
  • 出版社:OCR Subscription Services Inc
  • 摘要:There has been considerable recent interest in the mechanisms by which recessive mutations involving cancer genes may be expressed. We have developed an in vitro model to study this phenomenon in an endogenous autosomal gene in human cells. We have analyzed the molecular structural changes that lead to loss of heterozygosity at the thymidine kinase (tk) locus. The results indicate that expression of a recessive allele frequently occurs by loss of heterozygosity at that locus. Over 90% of spontaneous mutants at the tk locus arose by allele loss. The fraction of induced mutants that arose by this mechanism depended upon the inducing agent. Loss of the active tk allele was often accompanied by loss of linked genetic loci on the long arm of chromosome 17. These results suggest that large-scale chromosomal mutations resulting from events such as deletion or mitotic recombination may be an important mechanism for the expression of activated or mutated recessive genes in human cells. Such recessive mutations could involve oncogenes or other growth regulatory genes important in carcinogenesis. Full text Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (695K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References . 63 64 65 66
Loading...
联系我们|关于我们|网站声明
国家哲学社会科学文献中心版权所有