出版社：International Digital Organization for Scientific Information Publications
摘要：Bacillus or Bacillus-related genera were the most frequently documented isolates from Jordanian hotsprings in several earlier studies based on culture-dependent methods. This study aimed to review theabundance and composition of the genus Bacillus in Jordanian hot springs (Ma'in and Afra springs) using aculture-independent approach by Illumina MiSeq. The studied samples included three samples from Ma'in hotsprings (HS1, HS2 and HS3) and one sample from Afra hot springs (HS4). Relative to the domain Bacteria, theabundance of Bacillus was found to be very low: 2% (in HS1), 3% (in HS2), 0.5% (in HS3) and 1% (in HS4).However, the abundance in lower taxonomic ranks was found to be higher: at the level of phylum Firmicutes,the genus constitutes 7% in HS1, 41% in HS2, 31% in HS3 and 33% in HS4. Bacillus was found to dominatethe order Bacillales and class Bacilli only in case of HS2 (64%). In case of HS1, HS3 and HS4, the abundancewas 18, 47 and 39%, respectively. Finally, Bacillus was found to be dominant as compared to other generawithin the family Bacillaceae in case of HS2 (84%), HS3 (77%) and HS4 (61%) but not in HS1 (22%) which wasdominated by the genus Anoxybacillus (77%). In respect to genus composition, in all studied hot springs, thespecies B. firmus was found the dominant species. Nevertheless, other species with lesser abundances werealso detected like B. cereus, B. beringensis, B. weihenstephanensis, B. funiculus and B. Oceanisediminis.
