The purpose of this study was to develop and validate the Czech Version of the Difficulties in Emotion Regulation Scale Short Form (DERS-SF-CZ) and to examine its psychometric properties. The DERS-SF was translated into Czech with a back-translation procedure and then administered through an on-line survey. 364 participants (70 men, 294 women) completed the SCS-CZ, GAD-2 and PHQ-2. The confirmatory factor analysis confirmed the six-factor structure of the DERS-SF-CZ (χ²/df=1,84, SRMR=0.050, RMSEA=0.048, CFI=0.963, TLI=0.953, GFI=0.935). A single higher-order factor model was also confirmed (χ²/df=2,06, SRMR=0.064, RMSEA=0.054, CFI=0.951, TLI=0.942, GFI=0.923). Nevertheless, the awareness factor did not have any effect on the second order factor in this hierarchical model (β_aw=0,03 R²_a­w=0,001). The Cronbach’s alpha coefficients for total DERS-SF-CZ and subscales were as follows: DERS-SF-CZ (α=0,86), awareness (α=0,65), clarity (α=0,72), non-acceptance (α=0,83), goals (α=0,84), impulse (α=0,88), strategies (α=0,80). As expected, the DERS-SF-CZ demonstrated a significant positive correlation with anxiety and depressive symptoms questionnaires. The results support further use of the DERS-SF-CZ in clinical practice and research.