期刊名称：IOP Conference Series: Earth and Environmental Science
印刷版ISSN：1755-1307
电子版ISSN：1755-1315
出版年度：2018
卷号：170
期号：2
页码：022106
DOI：10.1088/1755-1315/170/2/022106
语种：English
出版社：IOP Publishing
摘要：Cellulose is the most abundant renewable resource in nature and can be used for the production of soluble sugars, biofuels and as raw materials for other important chemical products. Therefore, the development and research of multifunction's enzyme is of great significance for the degradation and utilization of cellulose. In this study, two-step DEAE-Sepharose FF weak anion exchange of fermentation supernatant of Bacillus subtilis LC-9 with different pH buffer as mobile phase was used to obtain the electrophoretic ally pure protein with a molecular weight of about 32 KDa, 25 KDa. The specific activity of filter paper comprehensively characterized by enzyme was 22.68U/mg, the purification fold was 33.35 and the recovery rate was 20.52%. In this study, the purified multifunction's enzyme not only has the activity of endoglucanase, xylanase, filter paper enzyme, exoglucanase and other cellulases, but also has the advantages of short enzyme producing time, simple purification method And other advantages, has a good prospect of industrialization.
