期刊名称：IOP Conference Series: Earth and Environmental Science
印刷版ISSN：1755-1307
电子版ISSN：1755-1315
出版年度：2018
卷号：170
期号：3
页码：032106
DOI：10.1088/1755-1315/170/3/032106
语种：English
出版社：IOP Publishing
摘要：This study aims to explore changes of community structures of AM (Arbuscular mycorrhizal) fungi after a long-term fertilization for 38 years in a brown soil. Soil samples (0-20 cm) were taken from six treatments in May 2016: (1) no fertilization (CK); (2) chemical N input (N); (3) chemical N and P input (NP); (4) chemical N, P and K input (NPK); (5) pig manure input (M); (6)pig manure, chemical N and P input (MNP). Community structures of AM (Arbuscular mycorrhizal) fungi were analyzed using PCR-DGGE, cloning and sequencing techniques. In seedling stage, the diversity of AM Fungi was the highest in non-fertilization treatment and the community structures of AM fungi was mainly divided into two groups.
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