Many educational institutions use a number of technologies in classrooms to promote the effectiveness of learning activities. In recent years, tablet PCs have become the primary of these technologies. Tablet PCs in particular play an important role in increasing the effectiveness and efficiency of the process of learning and teaching. In order to reveal this effect of tablet PCs on learning-teaching and to ensure that tablet PCs are used effectively by teachers, development of favorable attitudes by teachers towards computers and elimination of anxiety about using these devices are of utmost importance. The purpose of this study is to determine primary school and Turkish teachers’ anxiety level about using tablet PCs in the process of learning and teaching. To this end, the anxiety scale for tablet PC assisted teaching developed by the researcher was administrated to the teachers. In order to determine the teachers’ anxiety level about using tablet PC in teaching, cross-sectional survey technique was used in the research. According to the results of the research, Turkish teachers’ anxiety level about teaching with tablet PC was found to be higher than that of primary school teachers. Also, in both branches of teaching, females have higher anxiety level about teaching with tablet PC than males. The findings of the research suggest that teachers should be provided with adequate training in using tablet PCs and integration of tablet PCs into educational activities, and that the technical infrastructure should be arranged accordingly.