摘要：For proper enumeration of protozoa in activated sludge good
methodology is required. In this paper we present some remarks on
microscopic methodology of protozoa enumeration. This remarks concern
number of repetitions from one sample required to obtain good statistical
results as well as influence of sample aeration on number of found
protozoa. Presented data shows that at last 10 repetitions are required from
each sample to obtain low average confidence interval. Lower number of
repetitions leads to sharp increase in average confidence interval and loss
of statistical significance while higher number does not decrease average
confidence interval substantially. As measurements lasts for few hours lack
of sample’s aeration during measurement leads to detection of lower by
27% number of protozoa.
其他摘要：For proper enumeration of protozoa in activated sludge good methodology is required. In this paper we present some remarks on microscopic methodology of protozoa enumeration. This remarks concern number of repetitions from one sample required to obtain good statistical results as well as influence of sample aeration on number of found protozoa. Presented data shows that at last 10 repetitions are required from each sample to obtain low average confidence interval. Lower number of repetitions leads to sharp increase in average confidence interval and loss of statistical significance while higher number does not decrease average confidence interval substantially. As measurements lasts for few hours lack of sample’s aeration during measurement leads to detection of lower by 27% number of protozoa.
