摘要:The NGS method was used to investigate the biodiversity of
ARB dwelling in tap water, able to form biofilm on bullion media
supplemented with antibiotics: β-lactam, fluoroquinolone, 3rd generation
cephalosporin and tetracycline. Biofilms formed by cold- and hot-tap water
microorganisms were also examined. The presence of 10 ARGs in plasmid
DNA of cultivated biomass was tested using a standard PCR. The analyses
revealed that the most predominant families were Sphingomonadaceae,
Paenibacillaceae, Enterobacteriaceae, Bacillaceae
and Sphingomonadaceae in FQ, C3G, T, C-W and H-W samples,
respectively (explanations of abbreviations in the text). The ARGs did not
correlate with resistance phenotypes.
其他摘要:The NGS method was used to investigate the biodiversity of ARB dwelling in tap water, able to form biofilm on bullion media supplemented with antibiotics: β-lactam, fluoroquinolone, 3rd generation cephalosporin and tetracycline. Biofilms formed by cold-and hot-tap water microorganisms were also examined. The presence of 10 ARGs in plasmid DNA of cultivated biomass was tested using a standard PCR. The analyses revealed that the most predominant families were Sphingomonadaceae , Paenibacillaceae , Enterobacteriaceae , Bacillaceae and Sphingomonadaceae in FQ, C3G, T, C-W and H-W samples, respectively (explanations of abbreviations in the text). The ARGs did not correlate with resistance phenotypes.