首页    期刊浏览 2024年12月04日 星期三
登录注册

文章基本信息

  • 标题:Application of NGS for studying the antibiotic resistant microbiome in Wrocław tap water
  • 本地全文:下载
  • 作者:Agata Siedlecka ; Katarzyna Piekarska
  • 期刊名称:E3S Web of Conferences
  • 印刷版ISSN:2267-1242
  • 电子版ISSN:2267-1242
  • 出版年度:2018
  • 卷号:44
  • 页码:1-8
  • DOI:10.1051/e3sconf/20184400161
  • 出版社:EDP Sciences
  • 摘要:The NGS method was used to investigate the biodiversity of ARB dwelling in tap water, able to form biofilm on bullion media supplemented with antibiotics: β-lactam, fluoroquinolone, 3rd generation cephalosporin and tetracycline. Biofilms formed by cold- and hot-tap water microorganisms were also examined. The presence of 10 ARGs in plasmid DNA of cultivated biomass was tested using a standard PCR. The analyses revealed that the most predominant families were Sphingomonadaceae, Paenibacillaceae, Enterobacteriaceae, Bacillaceae and Sphingomonadaceae in FQ, C3G, T, C-W and H-W samples, respectively (explanations of abbreviations in the text). The ARGs did not correlate with resistance phenotypes.
  • 其他摘要:The NGS method was used to investigate the biodiversity of ARB dwelling in tap water, able to form biofilm on bullion media supplemented with antibiotics: β-lactam, fluoroquinolone, 3rd generation cephalosporin and tetracycline. Biofilms formed by cold-and hot-tap water microorganisms were also examined. The presence of 10 ARGs in plasmid DNA of cultivated biomass was tested using a standard PCR. The analyses revealed that the most predominant families were Sphingomonadaceae , Paenibacillaceae , Enterobacteriaceae , Bacillaceae and Sphingomonadaceae in FQ, C3G, T, C-W and H-W samples, respectively (explanations of abbreviations in the text). The ARGs did not correlate with resistance phenotypes.
国家哲学社会科学文献中心版权所有