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  • 标题:Methods of increasing the sensitivity of the haemagglutination inhibition test for rabies virus antibody
  • 本地全文:下载
  • 作者:K. Mannen ; K. Ishikawa ; J. Tachibana
  • 期刊名称:Bulletin of the World Health Organization
  • 印刷版ISSN:0042-9686
  • 出版年度:1984
  • 卷号:62
  • 期号:6
  • 页码:883-892
  • 语种:English
  • 出版社:World Health Organisation
  • 摘要:A new method for the removal of non-specific inhibitors of rabies virus haemagglutinin has been developed. Treatment with colloidal silicic acid (Aerosil) or with acetone plus Aerosil reduced the non-specific inhibitors in human, mouse, and dog sera to a level that was undetectable at the 1:4 starting dilution in the haemagglutination inhibition test. Bromelain-treated goose erythrocytes were much more susceptible to haemagglutination by rabies virus than were untreated erythrocytes, and the sensitivity of the haemagglutination inhibition test was considerably increased by using bromelain-treated erythrocytes. Low levels of antibodies in sera from immunized human subjects were detected with higher sensitivity by combining Aerosil treatment of the sera with the use of bromelain-treated goose erythrocytes in the haemagglutination inhibition test. Full text Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.0M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References . 883 884 885 886 887 889 890 891 892
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