标题：Recent developments in the assessment of the immune response to malaria, especially as related to vaccination: Malaria vaccination with irradiated sporozoites: serological evaluation of the antigen and antibody responses
摘要：Vaccination against Plasmodium falciparum with attenuated sporozoites is the goal of the US Navy's Malaria Vaccine Program. One requirement in the development of this vaccine is an immunological test to study the sporozoite antigen and immune responses it induces. Using an indirect fluorescent antibody test (IFAT) and P. berghei in the mouse or rat as a model, we have made significant progress toward this goal. Four antigens were detected in vaccine preparations: sporozoite-specific antigens, mosquito antigens, antigens on the sporozoite that are common to erythrocytic stages, and bovine serum albumm, an antigenic element of the isolation medium no longer employed. The IFAT was a reliable monitor of vaccination in a mouse and rat model in conjunction with protection to challenge. The test was a sensitive monitor of vaccine quality. Anamnestic responses to bites of infected mosquitos were detected in mice previously immunized with irradiated sporozoites. Full text Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (810K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References . 205 206 207 208 209
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