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  • 标题:Differentiation of variola, monkeypox, and vaccinia antisera by radioimmunoassay
  • 作者:Harriet D. Hutchinson ; Donald W. Ziegler ; David E. Wells
  • 期刊名称:Bulletin of the World Health Organization
  • 印刷版ISSN:0042-9686
  • 出版年度:1977
  • 卷号:55
  • 期号:5
  • 页码:613-623
  • 语种:English
  • 出版社:World Health Organisation
  • 摘要:Poxvirus antisera adsorbed with “homologous” and “heterologous” poxvirus-infected chorioallantoic membranes (CAM) were differentiated by solid-phase radioimmunoassay (RIA). Mixtures of the antiserum dilutions and infected CAM were added directly (without centrifugation) to poxvirus-infected CAM antigens affixed to wells of microtitration plates. The affixed antigens combined with unadsorbed antibodies, and the cross-reactive antigen—antibody complexes were removed by washing. The results showed that adsorption of an antiserum with variola-, vaccinia-, or uninfected-CAM antigen and subsequent reaction of each in RIA with monkeypox- and uninfected-CAM antigens allowed the identification of antivariola, antivaccinia, or antimonkeypox sera. Full text Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.4M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References . 613 614 615 616 617 618 619 620 621 622 623
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