摘要：Animal-infective forms of Trypanosoma brucei (Strain 427) were successfully propagated in HEPES-buffered RPMI 1640 medium in the presence of bovine fibroblast-like cells for over 310 days. The organisms grown in this system were morphologically identical to the long slender bloodstream forms, retained their infectivity for mammalian hosts, and displayed variant-antigen on their surface. Technical details for establishing such bloodstream form cultures are described in the present paper. Full text Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (813K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References . 405 406 407 408 409
