摘要：Successful primary isolation of chikungunya virus from clinical specimens was carried out using mouse embryo cell cultures. Parallel isolation studies using the classical intracerebral inoculation of suckling mice showed that both systems are about equally sensitive. However, the mouse embryo cultures seem to have certain advantages over the use of mice; these include economy of materials and time, precision, and a greater uniformity of reaction. Furthermore, the infective tissue-culture fluid provides a more convenient source of complement-fixing antigen. Compared with other cell cultures, mouse embryo cultures have the advantage of being readily available in all arbovirus laboratories. The method should be particularly useful in epidemic situations. Full text Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (402K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References . 487 488 489
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