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  • 标题:Non-tuberculosis mycobacteria in Africa: 3. Formamidase activity—its evaluation and practical application*
  • 本地全文:下载
  • 作者:M. P. Zykov ; H. Roulet
  • 期刊名称:Bulletin of the World Health Organization
  • 印刷版ISSN:0042-9686
  • 出版年度:1967
  • 卷号:37
  • 期号:6
  • 页码:947-951
  • 语种:English
  • 出版社:World Health Organisation
  • 摘要:The third study in a series on the prevalence of non-tuberculosis mycobacteria in Africa is devoted to the investigation of the formamidase activity of 288 cultures of mycobacteria, already typed by a battery of standard tests as pathogenic or atypical (184 strains) and saprophytic (104 strains). Of the latter, 96 (92.3%) were formamidase-positive, as compared with only 6 (3.3%) of the former. A close correlation was observed between the speed of growth on Löwenstein-Jensen medium and formamidase activity, 98 (96.1%) of the positive strains showing visible growth within 1-3 days. The relation between formamidase activity and growth on nutrient media was less clear-cut, however, and it was concluded that for the routine differentiation of saprophytic from other mycobacteria the formamidase test should be combined with simple tests such as speed of growth on L-J medium and ability to grow on nutrient media. Russel's method and Nessler's reagent for the detection of ammonia in the formamidase test were compared; the authors consider the former to be preferable, since the reaction is easier to read. Full text Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (486K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References . 947 948 949 950 951
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