摘要：The authors have attempted to prepare lysed BCG vaccines retaining the protective antigens of the BCG cell wall and yet eliciting in experimental animals limited sensitivity to the tuberculin substances, the advantage sought being to retain the usefulness of the tuberculin following vaccination as an indicator of superinfection. Experimental vaccines were prepared by growing and lysing BCG in both Dubos and Aldridge liquid media with glycine, lysosyme and lithium chloride; vaccines were also prepared by autolysis in Aldridge nitrogen-deficient, chemically defined medium. Glycine, lysozyme and lithium chloride significantly reduced both the protective and allergenic properties of the lysates. The best protection was afforded by BCG cells autolysed in Aldridge medium. Treatment of the BCG autolysates with lysozyme alone did not affect the protective capability of the vaccines and almost completely eliminated the allergenicity. It is suggested that these BCG lysates contain metabolically inactive cells, which do not multiply in vitro but may still provide sufficient antigen in vivo to ensure protection. Full text Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (890K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References . 973 974 975 976 977 978 979 980 981 982
