This paper investigates the action of nanoparticles ZnO (10 nm) and TiO₂ (30 nm) on growth of Saccharomyces cerevisiae CNMN-Y-20 yeast. Nanoparticles in concentration of 0,5; 1,0 and 5,0 mg/L in YPD medium did not modify significantly cell proliferation, biomass production, the carbohydrate content and the content of β-glucans at Saccharomyces cerevisiae CNMN-Y-20. Nanoparticles ZnO and TiO₂ contributed to the decrease in protein content, which demonstrated the appearance of the alterations of yeast cell membranes.