摘要：Bar-HRM is a hybrid method which combines DNA barcoding and High Resolution Melting analysis. It has proven to be a fast, cost-effective and reliable molecular approach for species identification and authentication. Here, three aspects of the use of Bar-HRM are focused on. First, Bar-HRM is used to discriminate between closely related Ophiocordyceps species. Second, identification of an unknown powder that is claimed to be Ophiocordyceps species using Bar-HRM. Third, authenticating the O . sinensis products sold on the market by the Bar-HRM. Results from HRM analyses with ITS primers shows that the two Ophiocordyceps species ( Ophiocordyceps sinensis and Ophiocordyceps militaris ) were easily differentiated. Also, an unknown sample was able to be identified in less time compared with using DNA barcoding alone. In addition, the substitution or adulteration of O . sinensis products sold on market was detected via Bar-HRM. The substitution or adulteration of inferior Ophiocordyceps species, particularly O . militaris in high price O . sinensis products has been a concern throughout Asia. Based on our results, the Bar-HRM was again proved to be a promising tool for species identification and authentication.
