标题：7. A NOVEL, COST –EFFECTIVE COVER SLIP HOLDER FOR IN SITU MICROSCOPY OF COVER SLIP CULTURES OF ACTINOMYCETES by RAVINDRAGOUDA PATIL1, G. JEYASEKARAN2 AND S. A. SHANMUGAM2
期刊名称：Liminalities : a Journal of Performance Studies
电子版ISSN：1557-2935
出版年度：2016
卷号：79
页码：60 to 64-60 to 64
语种：English
出版社：Liminalities
其他摘要：A novel, cost effective cover slip holder was designed and used. A modified cover slip culture method was developed and used. The routine smear preparation technique disturbs the morphological details of the aerial hyphae and spore chains of the actinomycete culture and makes it difficult for identification. The two coverslips with the inoculated medium sandwiched was incubated in a petri plate humid chamber. The cover slip culture was placed within the cover slip holder device and observed for the nature of substrate, aerial hyphae and the nature of spore chains. A novel, cost-effective cover-slip holder was designed in the laboratory using micro slides, glass cutter and silicone adhesive. The cover slip holder after completion, resembled the shape of roman numeral “II”. The cover slip culture was placed within the cover slip holder device and observed for the nature of substrate, aerial hyphae and the nature of spore chains.KEY WORD: Cover slip culture, Cover slip holder, Actinomycete, In situ microscopy, Mycelium, Spor.
