摘要：Purpose: In the present study, we aimed toidentify the effect of three increasing doses ofmost widely preservative, formaldehyde (FA) onthe urinary system using human embryonic kidneycells (HEK-293) in vitro.Materials and methods: The HEK-293 cellswere grown in Dulbecco’s Modified Eaglemedium (DMEM, Gibco, USA) supplementedwith 10% of fetal bovine serum, 100 IU/mlpenicillin and 100 μg/ml of streptomycin (Gibco,USA) using 25cm2flasks (Nunc, Denmark), in aCO2 incubator (Heal Force) at 37°C are treatedwith 19 % formaldehyde in DMEM supplementedwith 10% of fetal bovine serum to yield finalconcentrations of 0.05 μl/ml, 0.1 μl/ml, 0.5 μl/mland 1.0 μl/mlof 19% FA.Results: The result showed that statisticallysignificant dose dependent decrease in cellviability of HEK-293 cells with exposure toincreasing concentration of FA (0.05 µl/ml, 0.1µl/ml, 0.5 µl/ml and 1.0 µl/ml) on MTT assay.Extremely huge dose dependent DNA damagewith a dose dependent up regulation of mRNAexpression of pro-apoptotic iNOS and TNF-gene was observed when HEK-293 cells aretreated with the increasing concentration of FA. Inaddition, the protein expression levels of Bcl-2and Bax revealed that increasing concentration ofFA on HEK-293 cells down regulated the antiapoptoticBcl-2 and up regulated pro-apoptoticBax gene.Conclusions: Our result indicates that, high doseof FA is more dangerous than the low dose onHEK-293 cells with cellular damage.
关键词：Formaldehyde; Human embryonic;kidney cells; High and low dose
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