The mail goal of this paper was to analyse the environmental impact of agricultural practices in Valea Calugareasca viticultural ecosystem. Diversity characterizes the healthy state of ecosistems. The quantification of biodiversity was achieved in order to understand and compare the ecosystem community structure, based on the following indicators: Simpson biodiversity index, Shannon biodiversity index, equitability. They were applied five different variants of vines culture technology: (1) natural permanent grassing soil; (2) temporary natural grassing; (3) artificial grassing or sown; (4) cultivation of the strips between the rows with plants bees; (5) black furrow by plowing. The biodiversity at the level of useful and harmful insect species was influenced by the applied culture technology, this process been highlighted by the values of diversity indices. The two indices of diversity, Shannon and Simpson recorded the highest values in case of technological variants based on maintenance of soil by permanent natural grassing and artificial or sown grassing.