Since the expression of a high ability for in vitro regeneration and proliferation is a very important condition for any biotechnological approach for clonal propagation, the influence of genotype and culture medium composition on the number of shoots regenerated and their length in successive subcultures was investigated in Aronia melanocarpa (Michx.) Elliot cultivars ‘Melrom’ and ‘Nero’. Chokeberry cultivar ‘Nero’ showed a significantly higher ability of regeneration compared to the cultivar ‘Melrom’, the greatest number of shoots being obtained with the basal medium containing MS macroelements, LF microelements and LF vitamins, supplemented with 4,5 mg×dm-3 BA and 0,6 mg×dm-3 IBA.