Nature represents an inexhaustible resource for human people’s health and care. In the last couple of years, medicinal and aromatic plants (MAPs) became one of the main research fields in Romania due to their therapeutic properties. In this study, Cynara scolymus L. plant was selected, due to its usage as an alternative to synthetic drugs, considering costs and side effects. Furthermore, this plant leaves are a reach source of compounds with powerful benefits in liver protection, cholesterol inhibition, cardiovascular and kidney diseases, indigestion, rheumatism, with antioxidant and antibacterial properties. The present study consisted of chlorogenic acid identification by HPLC, total polyphenol and flavonoids content determination and antioxidant activity evaluation. Due to its wide spread, according to our preliminary study, this plant was harvested from Romanian territory and its extract was processed in optimal conditions. In this study, flavones and polyphenolic compounds were found to be abundant in Cynara scolymus L., particularly the chlorogenic acid.