摘要：Purpose: In spite of an extensive vaccination program, parvoviral infections still pose a majorthreat to the health of dogs.Materials and Methods: We isolated a novel canine parvovirus (CPV) strain from a dog withenteritis. Nucleotide and amino acid sequence analysis of the isolate showed that it is a noveltype 2b CPV with asparagine at the 426th position and valine at the 555th position in VP2. Todevelop a vaccine against CPV infection, we passaged the isolate 4 times in A72 cells.Results: The attenuated isolate conferred complete protection against lethal homologous CPVinfection in dogs such that they did not develop any clinical symptoms, and their antibody titersagainst CPV were significantly high at 7-11 days post infection.Conclusion: These results suggest that the virus isolate obtained after passaging can be developedas a novel vaccine against paroviral infection
关键词：Canine parvoviral infection; Vaccines; Novel type 2b; Dogs; Clinical isolate
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