摘要：Purpose: Neuraminidase (NA) of influenza virus contains stalk region that shows a great dealof variability in both amino acid sequence and length. In this paper, we investigated generationof recombinant influenza viruses that had hepatitis B virus (HBV) B cell epitopes in the NAstalk region as a dual vaccine candidate.Materials and Methods: We used the WSH-HK reassortant helper virus for rescue of recombinantinfluenza virus containing HBV epitopes and reverse genetic protocol based on the useof micrococcal nuclease-treated virus cores for reconstitution of ribonucleoproteins.Results: We successfully generated a chimeric influenza viruses which contained 22 aminoacid peptides in the stalk region derived from the surface and pre-surface protein HBV. Thegrowth kinetics of the recombinant viruses was investigated after infection of Madin-Darbycanine kidney (MDCK) and Madin-Darby bovine kidney (MDBK) cells and the rIV-BVPreS virusshowed higher titer than other viruses in MDCK cells. We also confirmed the presence of HBVepitopes in the chimeric viruses by enzyme-linked immunosorbent assay (ELISA) using anti-HBV polyclonal antibody. When the ratio of recombinant virus verse wild type virus was calculatedby ELISA, recombinant viruses exhibited 2 fold higher values than the wild type virus.Conclusion: These results suggest that chimeric influenza virus which contained foreign antigenscan be used as dual vaccine against both HBV and influenza viruses.
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