期刊名称：IOP Conference Series: Earth and Environmental Science
印刷版ISSN：1755-1307
电子版ISSN：1755-1315
出版年度：2018
卷号：186
期号：2
页码：012010
DOI：10.1088/1755-1315/186/2/012010
语种：English
出版社：IOP Publishing
摘要：This work studied a novel method to construct a mixed reverse micelle system and its application in extracting and purifying enzymes. Mixed reversed micellar extraction formed by biosurfactant surfactin and ionic surfactant polysorbate-80 was applied for extracting cellulase. 40% surfactin and 60% polysorbate-80 were found to be the best system. The activity and protein recovery were obtained with the best conditions: in forward extraction, surfactant concentration was 3.0 mM; pH 7.0; temperature 40°C; KCl 0.05 M. In backward extraction, pH 7.5; temperature 40°C; KCl 0.1 M.
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