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  • 标题:Comparative study of five Legume species based on De Novo Sequence Assembly and Annotation
  • 作者:Sagar S. Patel ; Dipti B. Shah ; Hetalkumar J. Panchal
  • 期刊名称:Computational Molecular Biology
  • 电子版ISSN:1927-5587
  • 出版年度:2014
  • 卷号:4
  • 期号:4
  • DOI:10.5376/cmb.2014.04.0009
  • 语种:English
  • 出版社:Sophia Publications
  • 摘要:Legume species are an important oilseed crop in tropical and subtropical regions of the world. Recently, next-generation sequencing technology, termed RNA-seq, has provided a powerful approach for analysing the Transcriptome. This study is focus on RNA-seq of five legume species which are Arachis hypogaea L. (The peanut) of SRR1212866, Cicer arietinum L. of SRR627764, Phaseolus vulgaris L. of SRR1283084, Trigonella foenum-graecum L. of SRR066197 and Vicia sativa L. of SRR403901 from NCBI database. Comparative study focuses on various important features like; reads were generated with N50, sequence assembly contigs which is further searched with known proteins and genes; among these, how many genes were annotated with gene ontology (GO) functional categories and sequences mapped to pathways by searching against the Kyoto Encyclopedia of Genes and Genomes pathway database (KEGG). These data will be useful for gene discovery and functional studies and the large number of transcripts reported in the current study will serve as a valuable genetic resource of these five legume species.
  • 关键词:De Novo assembly; Bioinformatics; Legume species; Sequence Assembly and Annotation
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