In this study, the effect of the harmony between organizational culture and values on job satisfaction is examined. Hierarchical regression analysis was applied to the data, which was obtained from the study conducted on 181 employees working in a private hospital in Istanbul. The result of the analysis shows that value-culture variation in which employees will have the highest job satisfaction is the traditionalist/conservative values-clan culture. The second most successful value-culture variation on job satisfaction is the impulsive/hedonistic values-adhocracy culture. In other words, it is predicted that job satisfaction will be high when an employee with traditionalist/conservative values works in an organization where clan culture is important, and an employee with impulsive/hedonistic values works in an organization where adhocracy culture is important. The most negative impacts on job satisfaction are impulsive/hedonistic values-clan culture and precautionary values-market culture. In other words, it can be said that an employee with impulsive/hedonistic values will be unhappy in clan culture, and an employee with precautionary values will be unhappy in market culture.