期刊名称：IOP Conference Series: Earth and Environmental Science
印刷版ISSN：1755-1307
电子版ISSN：1755-1315
出版年度：2018
卷号：199
期号：3
页码：032064
DOI：10.1088/1755-1315/199/3/032064
语种：English
出版社：IOP Publishing
摘要：The effects of lead stress on the dynamic changes of protein secondary structure and differential protein expression of sunflower seedlings in soil were studied using Fourier transform infrared spectroscopy (FTIR) and two-dimensional (2D) electrophoresis. Under lead stress treatment, the α-helices and β-sheets of protein secondary structure for sunflower seedlings were changed. The content changes, 110% increased in a-helices of roots and 40% declined in β-sheets of leaves, were observed, respectively. Furthermore, 11 differential protein spots were obtained by analyzing 2-DE gel images, while No. 9 were identified by MALDI-TOF/TOF MS, successfully.
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