摘要：Background: Previous studies revealed that selectins play key roles in homing of immune cells to inflamed tissues and lymphatic organs. L-selectins are expressed on immune cells and interact with P and E selectins to homing to the tissues, hence, the polymorphisms within the gene of L-selectins may are associated with alteration in its expression. Thus, the current cross-sectional analytical study has been designed to investigate the polymorphisms within L-selectin gene and their relation with visceral leishmaniasis (VL). Methods: This study was performed on 194 samples during 2004-2012.The PCR-SSP and immunoflorescence techniques were used to evaluate the L-selectins polymorphism and anti- Leishmania antibody titration, respectively, in 56, 74 and 64 seropositive VL patients (group 1) , seropositive healthy controls (group 2) and seronegative healthy controls ( group 3 ) . Results: The results showed that the genotypes ( P =0.711) and alleles ( P =0.679) within L-selectins gene (A/C) was not differ between groups. Our results also demonstrated that the genotypes within L-selectins in group 1 ( P =0.807) and 2 ( P =0.441) were not associated with the titration of anti-leishmania antibody. Conclusions: The results identified that the polymorphisms within L-selectins gene were not associated with VL and it may be concluded that these genotypes and alleles are unable to affect immune responses in VL patients.
其他摘要：Background: Previous studies revealed that selectins play key roles in homing of immune cells to inflamed tissues and lymphatic organs. L-selectins are expressed on immune cells and interact with P and E selectins to homing to the tissues, hence, the polymorphisms within the gene of L-selectins may are associated with alteration in its expression. Thus, the current cross-sectional analytical study has been designed to investigate the polymorphisms within L-selectin gene and their relation with visceral leishmaniasis (VL). Methods: This study was performed on 194 samples during 2004-2012.The PCR-SSP and immunoflorescence techniques were used to evaluate the L-selectins polymorphism and anti- Leishmania antibody titration, respectively, in 56, 74 and 64 seropositive VL patients (group 1) , seropositive healthy controls (group 2) and seronegative healthy controls ( group 3 ) . Results: The results showed that the genotypes ( P =0.711) and alleles ( P =0.679) within L-selectins gene (A/C) was not differ between groups. Our results also demonstrated that the genotypes within L-selectins in group 1 ( P =0.807) and 2 ( P =0.441) were not associated with the titration of anti-leishmania antibody. Conclusions: The results identified that the polymorphisms within L-selectins gene were not associated with VL and it may be concluded that these genotypes and alleles are unable to affect immune responses in VL patients.
Loading...
