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  • 标题:Multiplex Lateral Flow Immunoassay: An Overview of Strategies towards High-throughput Point-of-Need Testing
  • 作者:Laura Anfossi ; Laura Anfossi ; Fabio Di Nardo
  • 期刊名称:Biosensors
  • 电子版ISSN:2079-6374
  • 出版年度:2019
  • 卷号:9
  • 期号:1
  • 页码:2
  • DOI:10.3390/bios9010002
  • 语种:English
  • 出版社:MDPI Publishing
  • 摘要:Simultaneous measurement of different substances from a single sample is an emerging issue for achieving efficient and high-throughput detection in several fields of application. Although immunoanalytical techniques have well-established and prevailing advantages over alternative screening analytical platforms, one of the incoming challenges for immunoassay is exact multiplexing. Lateral flow immunoassay (LFIA) is a leading immunoanalytical technique for onsite analysis, thanks to its simplicity, rapidity, and cost-effectiveness. Moreover, LFIA architecture is adaptable to multiplexing, and is therefore a possible answer to the pressing demand of multiplexing point-of-need analysis. This review presents an overview of diverse approaches for multiplex LFIA, with a special focus on strategies based on new types of magnetic, fluorescent, and colored labels.
  • 关键词:immunoassay; rapid test; point-of-care testing; immunochromatographic test immunoassay ; rapid test ; point-of-care testing ; immunochromatographic test
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