期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2019
卷号：116
期号：3
页码：1053-1058
DOI：10.1073/pnas.1816371116
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Activation of most primary sensory neurons results in transduction currents that are carried by cations. One notable exception is the vertebrate olfactory receptor neuron (ORN), where the transduction current is carried largely by the anion Cl − . However, it remains unclear why ORNs use an anionic current for signal amplification. We have sought to provide clarification on this topic by studying the so far neglected dynamics of Na + , Ca 2 + , K + , and Cl − in the small space of olfactory cilia during an odorant response. Using computational modeling and simulations we compared the outcomes of signal amplification based on either Cl − or Na + currents. We found that amplification produced by Na + influx instead of a Cl − efflux is problematic for several reasons: First, the Na + current amplitude varies greatly, depending on mucosal ion concentration changes. Second, a Na + current leads to a large increase in the ciliary Na + concentration during an odorant response. This increase inhibits and even reverses Ca 2 + clearance by Na + / Ca 2 + / K + exchange, which is essential for response termination. Finally, a Na + current increases the ciliary osmotic pressure, which could cause swelling to damage the cilia. By contrast, a transduction pathway based on Cl − efflux circumvents these problems and renders the odorant response robust and reliable.
关键词：olfaction ; Ca-activated Cl current ; mathematical modeling ; electrodiffusion ; cilia ion dynamics
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