文章基本信息

标题：Expression and serological application of recombinant epitope-repeat protein carrying an immunodominant epitope of Newcastle disease virus nucleoprotein
作者：Gaikwad, Satish S. ; Gaikwad, Satish S. ; Lee, Hyun-Jeong 等
期刊名称：Clinical and Experimental Vaccine Research
印刷版ISSN：2287-3651
出版年度：2019
卷号：8
期号：1
页码：27-34
DOI：10.7774/cevr.2019.8.1.27
语种：English
出版社：KoreaMed Synapse
摘要：Purpose
The aim of the present study was to develop a serodiagnostic test for differentiation infected from vaccinated animal (DIVA) strategy accompanying the marker vaccine lacking an immunodominant epitope (IDE) of nucleoprotein of Newcastle disease virus (NDV).
Materials and Methods
Recombinant epitope-repeat protein (rERP) gene encoding eight repeats of the IDE sequence (ETQFLDLMRAVANSMR) by tetra-glycine linker was synthesized. Recombinant baculovirus carrying the rERP gene was generated to express the rERP in insect cells. Specificity and sensitivity of an indirect enzyme-linked immunosorbent assay (ELISA) employing the rERP was evaluated.
Results
The rERP with molecular weight of 20 kDa was successfully expressed by the recombinant baculovirus in an insect-baculovirus system. The rERP was antigenically functional as demonstrated by Western blotting. An indirect ELISA employing the rERP was developed and its specificity and sensitivity was determined. The ELISA test allowed discrimination of NDV infected sera from epitope deletion virus vaccinated sera.
Conclusion
The preliminary results represent rERP ELISA as a promising DIVA diagnostic tool.
关键词：Newcastle disease virus; Nucleoprotein; Epitope-repeat protein; Enzyme-Linked Immunosorbent assay; Differentiation infected from vaccinated animal