摘要：Malaria is the first killer as well as endemic disease in highland, lowland and coastal areas of Papua. In 2016 there were 160 thousands cases noted and 80% of the cases were caused by Plasmodium falciparum . Total sample for this study were provided by a clinical trial conducted from May to June 2018 in the regional hospital of Puncak Jaya, Wamena, located in the highland region of Papua. Positive blood samples was collected in a ethylene diaminetetra acetic acid (EDTA) tube. DNA was extracted using method of Geneaid Genomic DNA Mini Kit ( Blood/Cultured Cell ). The primary reactions used a set of primer corresponding to the conserved regions of block 2 of msp 1. The second reactions primer set targets specific allelic families of msp 1 (K1, MAD20 and RO33). All polymerase chain reaction amplicons were analyzed by electrophoresis in a 1.0% agarose gel. A total of 26 blood samples of positive P . falciparum were analyzed, 24 (93.2%) were successfully amplified for msp 1. The MAD20 allelic family was predominant with 20.8% (8/24), followed by the RO33 allelic family with 8.2% (2/24) and the K1 allelic family with 1/24 (4.2%). The frequency of single allele is 11/24 (45.8%) while multi-allele are 16/24 (66.7%) with K1/RO33, MAD20/RO33, K1/MAD20/RO33 respectively 2/24 (8.3%), 8/24 (33.3%) and 6/24 (25%).
