期刊名称:IOP Conference Series: Earth and Environmental Science
印刷版ISSN:1755-1307
电子版ISSN:1755-1315
出版年度:2019
卷号:237
期号:5
页码:052011
DOI:10.1088/1755-1315/237/5/052011
出版社:IOP Publishing
摘要:The research aimed to provide references for further study of gene transformation of blueberry, raspberry and cranberry in Agrobacterium-mediated transformation. Effects of carbenicillin on the growth and differentiation of various explants of three berries were studied, and the inhibitive effects of carbenicillin upon Agrobacterium tumefaciens also were studied. The results showed that the optimum carbenicillinon's concentration was 200~ 300 mg/kg in the differentiating stage of blueberry and raspberry, and the time of subculture was 7 days; the optimum carbenicillinon's concentration was 400 mg/kg in the differentiating stage of cranberry, and the time of subculture was 7 ~ 14 days; The optimum carbenicillin's concentration was under 200 mg/kg in the rooting stage of three berries.