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  • 标题:The Effects of Carbenicillin on Growth and Differentiation of Three Berries
  • 本地全文:下载
  • 作者:Ke Song ; Ke Song ; Wei Wang
  • 期刊名称:IOP Conference Series: Earth and Environmental Science
  • 印刷版ISSN:1755-1307
  • 电子版ISSN:1755-1315
  • 出版年度:2019
  • 卷号:237
  • 期号:5
  • 页码:052011
  • DOI:10.1088/1755-1315/237/5/052011
  • 出版社:IOP Publishing
  • 摘要:The research aimed to provide references for further study of gene transformation of blueberry, raspberry and cranberry in Agrobacterium-mediated transformation. Effects of carbenicillin on the growth and differentiation of various explants of three berries were studied, and the inhibitive effects of carbenicillin upon Agrobacterium tumefaciens also were studied. The results showed that the optimum carbenicillinon's concentration was 200~ 300 mg/kg in the differentiating stage of blueberry and raspberry, and the time of subculture was 7 days; the optimum carbenicillinon's concentration was 400 mg/kg in the differentiating stage of cranberry, and the time of subculture was 7 ~ 14 days; The optimum carbenicillin's concentration was under 200 mg/kg in the rooting stage of three berries.
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