出版社：National Nutrition and Food Technology Research Institute
摘要：Background and objective: Polyhydroxyalkanoates have drawn significant attention as
alternative to petroleum-based plastics; however, their industrial production is still hindered
by the costly feed materials. Co-generation of other high-value products in addition to
polyhydroxyalkanoate by the same microbial strains can be helpful in alleviating overall
production cost up to 50%. This study for the first time demonstrates that
polyhydroxyalkanoate and astaxanthin-rich carotenoids can be co-produced by Paracoccus sp.
LL1 using waste cooking oil as substrate.
Material and methods: The halophilic strain of Paracoccus sp. LL1 was grown under batch
fermentation using mineral media supplemented with 1% (v v-1
) waste cooking oil. Different
surfactants were used to improve substrate utilization. Polyhydroxyalkanoate obtained after
the fermentation was characterized by fluorescent microscopy, gas chromatography, and
Fourier Transform Infra-Red spectroscopy.
Results and conclusion: Oil as a substrate, led to 1.0 g l-1
poly (3-hydroxybutyrate-co-3-
hydroxyvalerate) with concomitant production of 0.89 mg l-1
of carotenoids after 96 h. An
enhancement of 2.7-folds in total cell dry mass was achieved when 0.1% (v v-1
) Tween-80
was used as surfactant for ease in oil metabolism. Paracoccus sp. LL1 has the potential to
serve as a single cell factory for bioconversion of cheap substrates into high value products.
Conflict of interest: The authors declare no conflict of interest.
其他摘要：Background and objective: Polyhydroxyalkanoates have drawn significant attention as alternative to petroleum-based plastics; however, their industrial production is still hindered by the costly feed materials. Co-generation of other high-value products in addition to polyhydroxyalkanoate by the same microbial strains can be helpful in alleviating overall production cost up to 50%. This study for the first time demonstrates that polyhydroxyalkanoate and astaxanthin-rich carotenoids can be co-produced by Paracoccus sp. LL1 using waste cooking oil as substrate. Material and methods: The halophilic strain of Paracoccus sp. LL1 was grown under batch fermentation using mineral media supplemented with 1% (v v-1) waste cooking oil. Different surfactants were used to improve substrate utilization. Polyhydroxyalkanoate obtained after the fermentation was characterized by fluorescent microscopy, gas chromatography, and Fourier Transform Infra-Red spectroscopy. Results and conclusion: Oil as a substrate, led to 1.0 g l-1 poly (3-hydroxybutyrate- co -3-hydroxyvalerate) with concomitant production of 0.89 mg l-1 of carotenoids after 96 h. An enhancement of 2.7-folds in total cell dry mass was achieved when 0.1% (v v-1) Tween-80 was used as surfactant for ease in oil metabolism. Paracoccus sp. LL1 has the potential to serve as a single cell factory for bioconversion of cheap substrates into high value products. Conflict of interest: The authors declare no conflict of interest.
